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An ortholog of farA of Aspergillus nidulans is implicated in the…

- - - - Involved in the transcriptional regulation of
        phospholipid biosynthetic genes in S. cerevisiae
    - - Membrane protein Scs2p in the absence of inositol, and
        Ino2p and Ino4p activate the transcription of phospholipid synthetic genes
  - - - Mediated by Yas1p
        and Yas2p through ARE1
  - - - CTF1b participate in the constitutive expression of cut2
      - CTF1a activates transcription of cut1 in the presence of cutin monomer
  - - - Represses the transcription of target genes by binding to Ino2p in the nucleus
- - - - induced in the presence of fatty acids
- - - - 2% (w/v) glucose
      - 2%(v/v) oleic acid or 2% (v/v) n-decane for liquid media
      - 2% (w/v) glycerol
    - - 0.2% fatty acids were dispersed by adding 1% Triton X100
      - . n-Alkanes were supplied by vapor
    - - The amplified fragments were digested with
        BamHI and XbaI or EcoR
      - cloned into the XbaI-EcoRI sites of
        pBluescript II SK(+)
      - obtain pULP1
      - digestion of pSAT4 with BamHI
        
        ADE1
        
        inserted
        into BamHI site of pULP1
        
        yielding the plasmid pDP1
      - digestion of pDP1 with XbaI and EcoRV
        
        introduced into the CXAU1
        
        mutant por1 strain
  - - - digested with XbaI and BglI
        
        cloned into XbaI-BamHI sites of pSUT5
    - - PAT1 promoter was amplified by using primers 5'
        -GCTCTAGAATATCTGCGGTGATCCCAGAC-30 and 50
        -GGAAGGCCTCATTGTGTGTGTGTGGGGAGGAT-3'
        
        digested with
        XbaI and StuI
        
        cloned into XbaI-StuI sites of pSUT5-LacZ
  - - - GeneRacer RLM-RACE
        kit (Invitrogen)
      - specific primers for 5'-RACE and 3'
        RACE were 5'-GCCTCTGACGTTCGCCGTTGCAGAAT-3' and 5'
        -CTGGTGGTGGGAGGGTCCCGAAGAC-3'
- - - - deletion of top gene which are YAL10F13321g , YALI0D17988g, and YALI0D10681g
      - translation product of YALI0F13321g and YALI0D10681g were included similarity with S.cereviase Pip2p in Y. lipolytica
    - - 0.2% fatty acid was dispersed in media by adding 1%
        Triton X-100.
      - no growth defect was observed on the
        medium containing oleic acid as a sole carbon source
      - whi;e S. cerevisiae, in which deletion of OAF1 or PIP2
        causes a severe growth defect on oleic acid
      - deletion of YALI0F13321g and YALI0D10681g, or
        YALI0D10681g and YALI0D17988g
        
        show no any not confer any defects on
        the growth on oleic acid
    - - suggested that the ORF of POR1 is 2751 bp in size and encodes a 916-amino acid protein
      - Two introns of 80 bp
        and 90 bp were found in POR1
      - Por1p has three domains
        
        Zn2Cys6 binuclear cluster domain
        
        specific transcription factor domain
        
        a glutamine-rich (Q-rich) domain
        
        first and second domains are conserved in
        orthologs of FarA
        
        Q-rich domains were found in Ctf1p
        of C. albicans
- - - - belonging to two distinct families.
    - - characterized in the budding yeast
        Saccharomyces cerevisiae
    - - appears to be conserved only in a subset of yeast species
    - - in which FarA and its paralog, FarB, belonging to another
        Zn2Cys6 transcription factor family
    - - in the genomes of wide variety of yeasts
        and filamentous fungi
    - - an ortholog of FarA in
        Candida albicans
        
        involved in fatty acid response
    - - engaged in the transcriptional
        regulation of cutinase genes
    - - found in the genome of Y. lipolytica
- - - - But, induction of genes by oleic acid diminish in Δpor1 strain