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An ortholog of farA of Aspergillus nidulans is implicated in the…

- - - - transcription factors, Yas1p and Yas2p,
    - - molecular mechanism of transcriptional regulation by fatty acids has been concealed in Y. lipolytica.
  - - - Zn2Cys6 transcription factors, Oaf1p and Pip2p, together with the C2H2 zinc finger protein Adr1p
        
        activate the transcription of target genes in
        response to fatty acids
      - this system appears to
        be conserved only in a subset of yeast specie
    - - control the transcription of genes involved in the metabolism of fatty acids
      - Orthologs of FarA and FarB are encoded in the genomes of wide variety of yeasts and filamentous fungi
        
        Ctf1p, an ortholog of FarA in Candida albicans, is involved in fatty acid response
        
        orthologs in Fusarium species, CTF1a and CTF1b of F. solani and Ctf1 of F. oxysporum , are engaged in the transcriptional regulation of cutinase genes
- - - - Analyzed their ability to assimilate fatty
        acid by observing their growth on oleic acid
      - To observe on fatty acids, 0.2% fatty acid was dispersed in media by adding 1% Triton X-100
      - No growth defect was observed on the
        medium containing oleic acid as a sole carbon source
        
        Marked contrast to S. cerevisiae, in which deletion of OAF1 or PIP2 causes a severe growth defect on oleic acid
      - Y. lipolytica has distinct mechanisms of transcriptional regulation of fatty acid utilization from S. cerevisiae, Oaf1p and Pip2p play vital roles in the growth on fatty acids
      - YALI0D12628g translation product as an ortholog of FarA of A. nidulans and Ctf1p of C. albicans in Y. lipolytica
        
        This gene designated as POR1 (Primary Oleate Regulator 1)
        
        Two introns of 80 bp
        and 90 bp were found in POR1
  - - - por1 strain grew normally on glycerol, glucose, and n-hexadecane, a relatively
      - por1 strain also grew well on n-decane, a shorter n-alkane, although slight growth impairment was observed
      - It exhibited severe growth defects on
        lauric acid and myristic acid
      - Por1p plays critical roles in fatty acid
        utilization in Y. lipolytica.
  - - - Northern blot analysis, the reporter activities were increased on oleic acid and n-decane in the wild type