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An ortholog of farA of Aspergillus nidulans is implicated in the…

- - - - grew
        normally
        
        glycerol
        
        glucose
        
        n-hexadecane
        
        n-decane
        
        slight growth impairment
      - growth defects
        
        lauric acid
        
        myristic acid
        
        oleic acid
        
        less profoundly
- - - - a fungal specific transcription factor domain (amino acid 327 to 422)
      - a glutamine-rich (Q-rich) domain (amino acid 661 to 742)
      - a fungal Zn2Cys6 binuclear cluster domain (amino acids 32 to 71)
- - - - no significant defects on oleic acid, less probable that orthologs of Oaf1p and/or Pip2p function in fatty acid response in Y. lipolytica
  - - - CTF1b
        participate in the constitutive expression of cut2, while CTF1a activates transcription of cut1 in the presence of cutin monome
  - - - Alternatively, it is possible that distinct regulatory mechanisms are involved in the fatty acid response
  - - - In Y. lipolytica, n-alkanes are oxidized to fatty acids, which are then metabolized through b-oxidation.
        
        transcription of these genes could in
        part be activated by fatty acids produced in the process of oxidation of n-alkanes by the action of Por1p
        
        considerable growth of the deletion mutant of POR1 on n-alkanes suggests that the expression of genes involved in b-oxidation and peroxisome proliferation is sufficient to support growth on n-alkanes even in the absence of Por1p
- - - - oleic acid
        
        activate beta-oxidation
        
        POT1
        
        peroxisomal 3-oxoacylCoA thiolase
        
        PAT1
        
        peroxisomal acetoacetyl-CoA thiolase
        
        POX2
        
        acyl-CoA oxidase
        
        Dpor1
        
        diminished induction of genes by oleic acid
  - - - type-1peroxisomal targeting signal (PTS1)
        
        induced by oleic acid in the wild-type
        
        less in the Dpor1
        
        deletion
        of POR1
        
        no apparent effect on the transcriptional activation
        of ALK1
        
        ALK1 were somehow increased in the Dpor1 cells on oleic acid compared to the wild-type
        cells
        
        P450ALK involved in terminal hydroxylation
        of n-alkane by n-decane
        
        POR1
        
        expressed on all carbon sources in the wild-type
        
        Ctf1p
        
        ortholog of FarA
        
        expression is induced by oleic acid
        
        POR1p
        
        reporter
        gene lacZ
        
        reporter activities increased on oleic acid & n-decane
        in wild-type
        
        essential transcription factor involved in transcriptional activation in response to fatty acids
        
        no elevation of b-galactosidase
        activity on oleic acid
        
        Slight defect in the activation of reporter gene
- - - - resulted in transcriptional activation of ALK1
  - - - Localizes the ER membrane by binding to phosphatidic acid(PA) and membrane protein(Scs2p) in the absence of inositol
      - In the presence of inositol, PA is used up for phosphatidylinositol synthesis, and Opi1p is released from the ER and repress the transcription of target genes by binding to Ino2p in the nucleus
    - - Activate the transcription of phospholipid synthetic genes
    - - localizes to the ER and nucleus in the presence on n-alkane
      - increases the possibility that Yas3p binds to the lipids or alkane metabolites in the ER
  - - - CGAGCCGA, which is consistent with the sequence in the palindrome 1 and 2 proposed as a binding site of CTF1a and CTF1b in the promoters of cutinase genes in F. solani