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The Metarhizium anisopliae Perilipin Homolog MPL1 Regulates Lipid…

- - - - N-terminal B-strands
      - B-strands and central three hydrophobic regionsthattargetandanchorPerAtoLDs
  - - - Yeast has lost the phosphorylation mechanism for removing perilipin-like proteins to access LDs
- - - - grown in insect hemolymph
  - - - monitor gene expression of Mpl1 mycelium from 36-h Sabouraud dextrose broth (SDB)
      - identify any inductive relationship with fatty acids
  - - - Duncan’s analysis of variance analysis
  - - - examine the potential involvement of MPL1 in this process
      - appressoria were induced on locust hind wings
- - - - Knocking out MPL1 ONLY impairs M.anisopliae to store lipid
    - - WT
        
        62% of LD diminish
        
        38% of LD migrate into germ tube apex
      - :small_red_triangle_down:MPL1
        
        thinner germ tubes - analogous to the lean phenotype of perilipin-deficient mice
        
        50% of germ tubes lack visible LDs
        
        50% have up to 3 LDs
        
        still able to transport residual LDs to hyphal tips
        
        MPL1 not crucial for mediating positioning droplets in Metarhizium
        
        contrast with Drosophila where peripilin regulates transportation of LDs
    - - WT
        
        LDs moved to differentiating hyphal tips
        
        enriched with LDs
      - :small_red_triangle:MPL1
        
        freq contained no LDs
    - - *Metarhizium produces variably shaped hypal bodies for dispersal in the hemocoele
      - Unlike conidia, LDs are dispersed throughout hyphal body cells, but as with other cell types, the :small_red_triangle:Mpl1 mutants have fewer
    - - regulation of lipid hemeostasis was also studies by assaying total intracellular lipids
        
        consistent with previous result
        
        WT has 2x more than the mutant (regardless of PDA, PDA plus O/insect cadavers
        
        7x more in WT than mutant (when in minimal media)
        
        indicates rapid depletion of residual stored lipid in the mutant