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The Metarhizium anisopliae Perilipin Homolog MPL1 Regulates Lipid…

- - - - Conidia and wild type mutant ere harvested in newly mycosed Manduca larvae. The samples were centrifuged, and the absorbance of supernatants was measured at 530 nm. Standard curve was generated
    - - single colony of pYes2Per transformed yeast cells was incubated overnight in YPD. Cells harvested from different media was washed 3x and adjusted to A600=1.0 with aterile water
      - Differences in lipid content between
        treatments were compared using the Duncan’s analysis of variance analysis
- - - - linked with the reduce ability to breach insect cuticle so that MPl1 is a pathogenecity determinant
- - - - A, RT-PCR analysis of Mpl1 expression in mycelia cultured for 6 h in water, minimal medium (MM), Sabouraud dextrose broth (SDB), bean root exudates (RE), 1%(w/v) Manduca cuticle (Cut), or hemolymph (HE).
      - B, time course study of Mpl1 expression in 36-h MM culture supplemented with oleic acid (600 M).
      - C, time course study of Mpl1 expression in conidia germinating in SDB.
      - D and E, co-localization of neutral lipids and MPL1 demonstrated by NR staining of GFP-MPL1 expressing cellsin conidia (D), mycelium (E) of M. anisopliae, and a budding yeast (S. cerevisiae) cell (F), respectively. BF, brightfield microscopy. Bar 5 m.