Please enable JavaScript.
Coggle requires JavaScript to display documents.
Genome-wide functional analysis reveals that infection-associated fungal…
Genome-wide functional analysis reveals that infection-associated fungal autophagy is necessary for rice blast disease
Discussion
-
-
-
-
-
-
-
-
-
ku70 mutant and showed that it significantly enhanced the frequency of homologous recombination to 80%
-
-
Phosphoprotein Atg13 is part of a multiprotein regulatory protein complex with Atg17, Vac8
Activate the Atg1 protein kinase in S. cerevisiae, necessary for induction
of autophagy
Reduction, but not elimination of autophagy
Atg18 is involved in recycling of Atg9, together with a
number of other proteins
-
Moatg11, Moatg24, Moatg26,
Moatg27, Moatg28, and Moatg29
-
-
Materials and Methods
1. Fungal Strains, Growth Conditions, and DNA Analysis.
Gel electrophoresis,restriction enzyme digestion, gel blots, and sequencing
-
-
-
-
-
RESULT
-
To test the role of gene in plant infection, the targeted gene was replace using ku70 mutant as recipient strain
-
-
the Atg mutant impaired in non selective autophagy were non pathogenic or highly reduce in virulence
-
-
-
complementation analysis was performed on a subset of atg mutant and and all cases tested led to restoration
-
INTRODUCTION
-
To define whether autophagy carried out by M. oryzae during plant infection is a selective or a nonselective form of autophagy
-
-
-
The autophagosome expands and then fuses with a vacuole, the lytic compartment (lysosome equivalent) of fungal cells, sequestering its contents and inner membrane for degradation by hydrolase
Selective forms of autophagy degrade peroxisomes (pexophagy), mitochondria, and endoplasmic reticulum or can occur during the biosynthetic cytoplasm-to-vacuole-targeting pathyway
-
Decided to adopt a genome-wide approach in which we would systematically analyze the autophagic machinery of M. oryzae
To determine why fungal autophagy is necessary for rice blast disease and to define which type of autophagy takes place during plant infection
-