Chapter 8
Gibbs Free Energy
ΔG=ΔH-TΔS
ΔG: Available Energy
ΔH: Enthalpy (will decrease in spontaneous reaction)
ΔS: Entropy (will increase in spontaneous reaction)
T: Temperature (can affect spontaneous reactions)
Terms
Entropy: Disorder
Enthalpy: Order
Anabolic: Building bigger molecules
Catabolic: Breaking molecules down
Metabolism: Total chemical reactions that occur in an organism
Temperature: Average movement of molecules
Heat: Total movement of molecules
Endergonic: Taking in energy
Exergonic: Releasing energy
Endothermic: Taking in heat
Exothermic: Releasing heat
Laws of Thermodynamics
2nd Law of Thermodynamics: Every time we convert energy, we create entropy in the universe.
1st Law of Thermodynamics: Energy cannot be created nor destroyed, only transferred.
Enthalpy decreases: Free energy goes down
ATP
Enthalpy increases: Free energy goes up
Entropy decreases: Free energy goes up
Entropy increases: Free energy goes down
ATP has a sugar with a nitrogen base adenine, along with three phosphate groups.
ATP can be used for three types of cellular work, being transport, mechanical, and chemical
Hydrolysis of ATP is when the bond of a phosphate group breaks by a water molecules (turns into ADP)
Mechanical: Transfer phosphate to motor proteins to allow cells to contract
Transport: Pumps substances across membrane
Chemical: ATP used to cause chemical reactions
Phosphorylation is the adding back of a phosphate group
Enzymes
Enzymes are proteins that end with "-ase"
Active Site: Where the reaction occurs
Enzyme-substrate complex: Temporary molecule formed when substrate and enzyme come together
Substrate: Substance an enzyme acts upon
Cofactors: Endergonic, non-protein, that affects enzymes by giving it the right shape
Coenzymes: non-proteins that turn substrates into products
Competitive inhibitor: Blocks active site so substrate cannot fit
Non-competitive inhibitor: Does not stick to active site but disrupts hydrogen bonds
Allosteric activation: Changing enzyme to the right shape
Allosteric inactivation: Changing enzyme to the incorrect shape
Cooperativity: Shape of the enzyme is altered by the substrate
Feedback inhibition: The end product is able to regulate the production of enzymes
Cellular Respiration
Photosynthesis
6CO2+6H20--->(light) C6H12O6+6O2
C6H12O6+6O2--->6CO2+6H2O+energy
The goal of this process is to create glucose which will provide energy for plants and eventually all living things on Earth.
The goal of cellular respiration is to use glucose to make ATP which will then provide cells the energy they need.
Photosynthesis relates to Gibbs Free Energy because it is endergonic and anabolic
Cellular Respiration relates to Gibbs Free Energy because it is exergonic and catabolic
Glycolysis: Uses 2 ATP to break glucose in 2 pyruvic acid molecules
-Gain 3 carbons after breaking glucose down
-Covalent bonds are broken (catabolic)
-Converts 4 ADP into 4 ATP and 2 NAD+ into 2 NADH (endergonic)
Reactants: 1 glucose, 2 ATP
Products: 2 pyruvate, 4 ATP, 2 NADH
Intermediate Step: The reactants are 2 pyruvates with 3 carbons each
-(catabolic) 1 carbon released for each pyruvate, then each carbon binds to oxygen and electrons, CO2 released by organism when it respires
-(catabolic) 2 carbons remain for each acetyl-CoA, which becomes a product
-2 NAD+ are converted into 2 NADH (endergonic)
Kreb's Cycle: 2 carbon Acetyl-CoA binds to Oxaloacetic Acid, which has 4 carbons, to make citric acid, a 6 carbon molecule
-citric acid is then broken down in which 2 carbon atoms are removed and excreted as carbon dioxide
-the remaining 4 carbon molecule binds to another Acetyl-CoA to continue the cycle
-each turn of the cycle produces 1 ATP, 3 NADH, and 1 FADH2
-one glucose can power two turns of the cycle
The Electron Transport Chain and Oxidative Phosphorylation: After glycolysis and two turns of the Kreb's Cycle, one glucose has provided 4 ATP, 10 NADH, and 2 FADH2
-The chain is a series of enzymes in the cristae of the mitochondria. NADH and FADH2 provide high energy electrons to the electron transport chain, which allow hydrogen ions to be pumped through the inner membrane of the mitochondria. The high concentration of hydrogen ions bind to oxygen to create water molecules which become a waste product
-ATP synthesis converts ADP into ATP (anabolic)(endergonic). This is powered by the movement of hydrogen ions throughout the inner membrane of the mitochondria.
-1 NADH can phosphorylate 3 ADPs into ATP, and 1 FADH2 can phosphorylate 2 ADPs into ATP
-The final products are typically 32-34 ATP
Electron Transport Chain: enzymes embedded in the cristae that pass electrons from one enzyme to another
Cristae is the inner membrane of the mitochondria that houses the electron transport chain
Light Reactions at Photosystems II:
-In the thylakoid, light and H2O are taken in. H2O is broken down by Photosystem II into electrons and hydrogen ions, with oxygen as a waste product. (catabolic/exergonic)
-Electrons are passed from Photosystem II into the cytochrome complex
-Cytochrome complex passed H+ ions from stroma into thylakoid. Electrons pass through
Light Reactions at Photosystem I:
-Electrons are taken from water using light energy in photosystem 1, with oxygen excreted
-Hydrogen ions pumped into thylakoid against concentration gradient (catabolic/exergonic)
-electrons, hydrogen ions, and NADP+ go to NADP+ reductionase, with NADPH created
-Hydrogen ions escape through ATP synthase that turns ADP into ATP
Dark Reactions: Cycle is down twice, located in stroma
-starts with 3CO2 molecules and 3RuBP molecules, which interacts with rubisco
-6PGA created (exergonic)
-uses 6ATP and 6NADPH to get to G3P, which is half a glucose combines with a G3P from the previous cycle to create 1 glucose
-remaining continue into the cycle again
Thylakoid: The site of light reactions in the chloroplast, arranged in stacks
Stroma: Where dark reactions occur to produce glucose