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The glyoxylate cycle is required for fungal virulence (The virulence of…

- - - - The macrophages and associated yeast were removed by scraping, and pooled by centrifugation for 1min at 500g
        
        Cell number and viability are determined (using
        methylene blue) by microscopy
        
        The cell mixture was washed twice with ice-cold water to
        lyse the mammalian cells
        
        The resulting cell pellet consisting mostly of yeast cells was
        frozen at -80 degree celcius
- - - - central regulatory point in gluconeogenesis
- - - - glyoxylate cycle is the only route to the synthesis of glucose in this environment.
- - - - C.albicans are grown in media containing oleic acid as sole C source
        
        Wild-type uses oleic acid as well as acetate
        
        Isocitrate lyase mutant is unable to metabolize oleic acid
      - Glyoxylate pathway is necessary for virulence, but is not sufficient
        
        Both Saccharomyces and Candida induce glyoxylate cycle on macrophage contact, but only Candida is virulent
  - - - Prevent survival of these pathogens inside the macrophage