INTRO to METABOLISM & CELLULAR RESPIRATION/ FERMENTATION

CELLULAR RESPIRATION/ FERMENTATION

INTRO TO METABOLISM

TYPES OF PATHWAYS

CATABOLIC PATHWAYS

ANABOLIC PATHWAYS

METABOLIC PATHWAYS

begins with a specific molecule, which is then altered in a series of defined steps, resulting in a certain product

metabolic pathways release energy by breaking down complex molecules to simpler compounds

major pathway of catabolism is cellular respiration

consume energy to build complicated molecules from simpler ones; they are some- times called biosynthetic pathways

example: the synthesis of an amino acid from a simple molecule to a protein from an amino acid

FORMS OF ENERGY

KINETIC ENERGY

THERMAL ENERGY

energy is the capacity to cause change

POTENTIAL ENERGY

CHEMICAL ENERGY

energy associated with the relative motion of an object

example:the motion of the cue stick to push the cue ball, which in turn moves the other balls

is kinetic energy associated with the random movement of atoms or molecules; thermal energy in transfer from one object to another is called heat

light would be an example

an object not presently moving may still possess energy

energy is not kinetic

it is energy that matter possesses because of its location or structure

example:Water behind a dam, for instance, possesses energy because of its altitude above sea level

refers to the potential energy available for release in a chemical reaction

ATP POWERS CELLULAR WORK BY COUPLING EXERGONIC REACTIONS TO ENDERGONIC REACTIONS

cells do chemical, transport, and mechanical work

cells manage their energy resources to do work by energy coupling, the use of an exergonic process to drive an endergonic one

ATP (adenosine triphosphate) is responsible for mediating most energy coupling in cells

STRUCTURE AND HYDROLYSIS OF ATP

during chemical work, the pushing of endergonic reactions that would not occur spontaneously, such as the synthesis of polymers from monomers

during transport work, the pumping of substances across membranes against the direction of spontaneous movement

during mechanical work, the contraction of muscle cells, and
the movement of chromosomes during cellular reproduction

ATP contains sugar ribose, with the nitrogenous base adenine and a chain of three phosphate groups (the triphosphate group) bonded to it

with help of some enzymes, cells are able to use energy released by ATP hydrolysis directly to drive chemical reactions

the key to coupling exergonic and endergonic reactions is the formation of as phosphorylated intermediate

ENZYMES SPEED UP METABOLIC REACTIONS BY LOWERING ENERGY BARRIERS

enzymes are a macromolecule that act as a catalyst, a chemical agent that speeds up a reaction without being consumed by the reaction

without enzymes chemical traffic through the pathways of metabolism would become terribly congested because many chemical reactions would take such a long time

enzymes lower the activation energy of a chemical reaction so it will speed up the reaction.

the reactant of an enzyme acts on is referred to as the enzyme’s substrate

enzyme binds to its substrate (or sub- strates, when there are two or more reactants), forming an enzyme-substrate complex

while enzyme and substrate are joined, the catalytic action of the enzyme converts the substrate to the product (or products) of the reaction

Only a restricted region of the enzyme molecule actually binds to the substrate, this region is the active site

CATABOLIC PATHWAYS AND PRODUCTION of ATP

one catabolic process, fermentation, is a partial degradation of sugars or other organic fuel that occurs without the use of oxygen

the most efficient catabolic pathway is aerobic respiration, in which oxygen is consumed as a reactant along with the organic fuel

most eukaryotic and many prokaryotic organisms can carry out aerobic respiration

cellular respiration includes both aerobic and anaerobic processes

the breakdown of glucose is exergonic

ELECTRON TRANSPORT CHAIN

consists of a number of molecules, mostly proteins, built into the inner membrane of the mitochondria of eukaryotic cells (and the plasma membrane of respiring prokaryotes)

electrons removed from glucose are shuttled by NADH to the “top,” higher-energy end of the chain

at the “bottom,” lower-energy end, O2 captures these electrons along with hydrogen nuclei (H+), forming water

electron transfer from NADH to oxygen is an exergonic reaction

instead of this energy being released and wasted in a single explosive step, electrons cascade down the chain from one carrier molecule to the next in a series of redox reactions, losing a small amount of energy with each step until they finally reach oxygen, the terminal elec- tron acceptor, which has a very great affinity for electrons

GLYCOLYSIS

occurs in the cytosol

begins the degrada- tion process by breaking glucose into two molecules of a compound called pyruvate

Some of the steps of glycolysis and the citric acid cycle are redox reactions in which dehydrogenases transfer electrons from substrates to NAD+ or the related electron carrier FAD, forming NADH or FADH2

TRANSITION REACTION

location: cytoplasm->mitochondria

no oxygen required

Reactants: 2 pyruvate acid, 2NAD+ from ETS, 2 coenzyme A

Products: 2CO2, 2NADH- go to ETS, 2 Acetyl CoA

KREBS CYCLE

Location: mitochondrial matrix

no oxygen required

Reactants: 2 Acetyl CoA, 6 NAD+ from ETS, 2 FAD fromETS, 2 ADP + 2 P

Products:4 CO2, 6 NADH go to ETS, 2 FADH2 go to ETS, 2 ATP