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General principles chromatography (definition (chromatogpraphy, mobile…
General principles chromatography
definition
chromatogpraphy
mobile phase
stationary phase
supporting medium
elution
chromatogram
separation process
plate theory
in distillation
in packed column
adv/dis
rate theory
adv/dis
depend on 2 factors
dy/dx in retention of solutes
sufficiently narrow width solute peaks
dy/dx plate theory n rate theory
migration rate of solute
dist constant (partition coe)
retention time
retention factor/capacity factor, k'
retention vol
selectivity factor/relative retention
method describe column eff
quantitative measure
no theoretical plate,N
height equivalent to theoretical plate,HETP/plate height,H
ideal vs non ideal chroma
Factor effect column eff
eddy diffusion
mp mass transfer
stagnant mp mss transfer
sp mass transfer
longitudinal diffusion
Van deemter eq
reduce band broadening
9.resolution
effect retention factor n selectivity factor on resolution
effect on retention time
factor increase resolution
analysis
qualitative
2 jenis,
analytical chroma
preparative
quantitative