Case Study: A young boy appears to have broken several bones in multiple locations. He eats junk food & he is lactose intolerant. Its questioned as to how his diet relates to his injury.

Upstream Causes

Downstream Effects

Direct

Indirect

Direct

Indirect

Problem # 2

Problem #1

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Background

Bones

Broken bone injury

Occurred in a boy, the age of 12

He ran & fell forward

Landing on right arm

Broken bones & their locations

Part of the humerus is protruding through the skin

Factors that contributed to easy breaks in such a young boy

Bones are classified by their shape & location

Bone functions

Hormone production

Blood cell formation

Mineral & growth factor storage

Triglyceride storage

Anchorage

Support

Protection

Only in certain areas

Such as

Vertebrae surround the spinal cord

Rib cage protects vital organs

Fused bones of skull protect brain

The femur for example does not protect anything

Skeletal muscles use bones as levers to move body

Anatomy of bones classified by shape

Divided into 2 groups

Axial skeleton

Appendicular

Ex: bone stores Ca & P, along w/growth hormone

Which is then released into the bloodstream as the body needs it

Hematopoiesis ( blood cell formation) occurs in red marrow of certain bones

Fat can be stored in bone as yellow marrow

For an energy source

Bone produce osteocalcin (hormone)

Which regulate

Glucose homeostasis

Energy expenditure

Insulin secretion

The skeleton provides a framework for the body to be used by muscles

Includes

Vertebral column

Rib cage

Skull

Includes

Bones of upper & lower limbs

Girdles (shoulder & hip bones)

Short bones

Flat bones

Irregular bones

Special type of short bone

Sesamoid bones

Shape

Long bones

Shape

Consists of a shaft & 2 ends

Ex: All limbs except patella, wrist, & ankle bones

Known for elongated shape, not overall size

Other ex: the 3 bones in the fingers (phalanges)

Cubed

Ex: Bones of wrist & ankle

Form in a tendon

Vary in size & # in individuals

Ex: Patella

Shape

Flat

A bit curved

Thin

Example

scapulae

Ribs

Sternum

Most cranial bones

Shape

They have complicated shapes

Examples

Vertebrae

Hip bones

Longer than they are wide

Characteristics of bone

They are organs

Structure

Contain

Epithelial in blood vessels in the bone

Dense connective tissue covering their external surface

Cartilage in their articular cartilages

Nervous tissue in their nerves

Smooth muscle around blood vessels

Sometimes muscle fibers

3 bone structural levels

Gross anatomy

Spongy bone

Compact bone

Internal layer - AKA -> trabecular bone

The honeycomb of small flat pieces -> trabeculae

Thin plates of spongy bone -> Diploe

The network of spongy material

Importance: Adult's way of making blood in bones

Anatomy

All contain 3 general structures

Bone ends

Membranes

Shaft

AKA -> Epiphyses

AKA -> Diaphysis

Forming the long axis

Constructed of

A collar of compact bone surrounding a medullary cavity

Contains NO bone tissue yet contains yellow marrow

Between the marrow & compact bone -> spongy bone

Spongy bone resides here

Between the epiphyses & diaphysis

Epiphyseal line

A remnant of the epiphyseal plate -> "Growth plate"

A disc of hyaline cartilage

Function: Grows during childhood to lengthen bones

Both are called metaphysis

Periosteum

Double layer membrane

Endosteum

Covers external part of bone EXCEPT joint surfaces

Containing nerve fibers, perforating fibers, & blood vessels

Outer layer composition: Dense IRREGULAR connective tissue

Inner osteogenic layer contains osteoprogenitor cells

Stem cells that give rise to most bone cells

Containing

Osteoblasts

Osteoclasts

Bone-forming cells

Bone-destroying cells

Within the bone

Covers trabeculae & lines canals that pass through compact bone

Containing

Same cells as the inner layer of periosteum

Osteoprogenitor cells

Other structural components

Well vascularized

Main vessels in diaphysis

Nutrient artery

Nutrient vein

Nutrient foramen

Nerves accompany blood vessels here also

Hematopoietic tissue

Blood forming tissue

AKA red marrow

In infants

In adults

Location

Medullary cavity of diaphysis

All areas of spongy bone

Red marrow is replaced w / yellow marrow

Red marrow is only found in spongy bone cavities between trabeculae of spongy bone -> diploe

Such as

Heads of

Sternum

Ribs

Flat bones of skull

Clavicles

Scapulae

Hip bones

Vertebrae

Femur

Humerus

Microscopic anatomy

5 major cell types in bone tissue

Osteoblast

Osteocytes

Osteoprogenitor cells

Bone lining cells

Osteoclasts

Mitotically active stem cells

Location

Periosteum

Endosteum

Function: When stimulated, some of these become

Osteoblast

Which can become

Osteocytes

Function: Bone forming cells

Shape

In growing bones

Also actively mitotic

That secrete bone matrix

Shape

When actively depositing matrix

Cube shaped

When inactive

Resemble flattened osteoprogenitor cells

Shape

Spidery looking & conform to shape of lacunae

Mature bone cells

Function

Flattened or squamous cells

Shape

Monitor & maintain the bone matrix

Act as sensors

Respond to stimuli

Communicate this info -> osteoclasts & osteoblasts

In bone remodeling

Trigger bone remodeling to maintain calcium homeostasis

Flat cells

Location: Bone surfaces where bone remodeling occurs

Function: Thought to help maintain matrix

Shape

Giant multinucleated cells

They have a ruffled border -> Increase surface area

Location: At sites for bone resorption (breaking down bone)

Function: Break down bone

External dense outer layer of bone

Chemical composition of bone (Made of 2 different things in matrix)

Soft organic components

Includes

Osteoid

Allowing it to resist stretch

Hard inorganic components

Includes

Mineral salts AKA hydroxyapatites

Allowing it to resist compression

Cells

Osteoprogenitor cells

Osteoblasts

Osteocytes

Bone lining cells

Osteoclasts

The protein part (collagen)

Makes up 1/3 of matrix

Secreted by osteoblasts

Bone formation

Endochondral ossification

Process

Intramembranous ossification

Bones formed

1) Hyaline cartilage is surrounded by perichondrium initially

Mesenchymal cells become osteoblasts

Perichondrium becomes periosteum

A collar bone forms when the osteoblasts secrete osteoid against the hyaline cartilage diaphysis

2) Cartilage calcifies in center of diaphysis & develops into cavities -> Area of deteriorating cartilage matrix

3) Periosteal bud invades cavities -> Spongy bone replaces cartilage

4) Diaphysis elongates & medullary cavity forms

Characteristics

Begins in 2nd month of development

In the collar bone, condrocytes in shaft enlarge -> Primary ossification center

Secondary ossification centers appear in epiphyses

Month 3 of development

Week 9 until birth

5) The epiphyses ossify

Then ossification is complete, hyaline cartilage remains only in epiphyseal plates & articular cartilages

Bones formed

Mainly long bones w/some others

Flat bones

Such as

Cranial bones

Clavicles

Characteristics

Begins at week 8 of development

Process

1) Ossification begins w / a membrane of fibrous connective tissue

2) Osteoid, secreted by osteoblasts, begins to calcify in days time

Mesenchymal cells cluster & differentiate into osteoblasts

3) Immature spongy bone & periosteum form

Trapped osteoblasts become osteocytes

Osteoblasts on outside make compact bone on surfaces

4) Red marrow develops

Whats left over from the connective tissue is what forms the periosteum

Postnatal bone growth

2 Ways bones grow

Appositional growth

Interstitial growth AKA longitudinal growth

All bones that grow in thickness

Long bones lengthen by

Growth of the epiphyseal plate cartilage & its replacement by bone

Occurs within the tissue

Occurs on the outside of bone by means of addition of it

Process

4) Ossification zone

3) Calcification zone

Goal

2) Hypertrophic zone

1) Proliferation zone

Cartilage cells (condroblasts) undergo mitosis

Pushing the epiphysis away from diaphysis

Which lengthens the long bone

The older condrocytes closer to diaphysis enlarge

Their lucunae erode & enlarge

Leaving interconnecting spaces

Adding Ca in order that the matrix becomes calcified

Chondrocytes die

Matrix deteriorates

Blood vessels invade

Osteoclasts partly erode the cartilage spicules created from the calcification process

Then osteoblasts cover them with new bone

Spongy bone replaces them

Osteoclasts digest spicules & the medullary cavity lengthens

Push the epiphysis up & add bone to diaphysis

Which pushes the the epiphyses further apart

Process

1) Osteoblasts in periosteum add more matrix layers on the external surface of bone

2) At the same time that step one is occurring, in the endosteal surface of the diaphysis, osteoclast remove bone

Goal: The outside gains more layers & the inside loses layers to widen bone w/out making it too heavy

Bone remodeling

Hormones responsible in bone remodeling

Calcitonin

PTH

Stimulate osteoclast to break down bone matrix

Produced by thyroid to help slow down osteoclast activity

Hormones responsible in bone growth

Thyroid hormones

Estrogens & testosterone

Growth hormone

Masculinization or feminization of parts of skeleton depend on theses levels

Modulate the activity of growth hormone

Released by anterior pituitary gland

Process

Characteristics

Remodeling replaces 5-10% of our skeleton every yr

Our entire skeleton is replaced about every 10 yrs

Spongy - Every 3-4 yrs

Compact - Every 10 yrs

2 main steps involved

Bone resorption

Bone deposition

Includes removal of old matrix w/replacement of new

Occurs: From embryo -> death

Osteoblasts deposit Ca & proteins throughout osteoid

Creating calcified bone matrix

Increase blood-Ca levels

In adults, both of these work at the same rate

Goal of both of these

To regulate BLOOD-Ca LEVELS

Maintain Ca homeostasis

Keeping bone strong

PTH is released

Osteoclasts remove the minerals & proteins

Fracture classification

Position of bone ends

Completeness of break

Whether the bone ends penetrate the skin

Displaced

Nondisplaced fractures

Bone ends are lined in position

Bone ends are not lined in position

The bone is broken all the way through

The bone is an open (compound) fracture

Common types of fractures

Epiphyseal break

Spiral

Compression

Greenstick

Comminuted

Depressed

Bones shatter in many pieces

Ex: In elderly

Bone is crushed

Ex: Calcaneous shatters if you land on your heels too hard

Twisted break

Ex: Machinery accidents or abused child

Epiphysis separates from diaphysis along the plate

Is a serious injury b/c it could effect growth

Growth hormone & steroids are possibles -> mitosis

Broken bone is pressed inward

Ex: skull fracture

Bone breaks incompletely (only one side breaks)

Common in kids w/ more organic matrix

Fracture Treatment & Repair

Treatment

Begins w/ reduction

Realignment of broken bones

Stages of bone healing in a simple fracture

Closed external reduction

Open internal reduction

Physician's hand put bone ends into position

Bone ends are secured together surgically w/screws & plates

After reduced

Immobilized by

Traction

Cast

Time it takes adults to heal (small - medium )

6-8 wks

2) Fibrocartilaginous callus forms

3) Spongy bone forms -> Bony callus forms

1) Lots of blood pulling -> Hematoma forms

4) Bone remodeling occurs

When a bone breaks, blood vessels in bone are torn

The hemorroraged blood clots forming hematoma

Tissue becomes swollen

Blood vessels grow into new clot

Fibroblast & condroblasts invade the fracture site

Bony callus is removed

Compact bone is laid down

Fibrocartilaginous callus spans the break & connects the broken bone ends

Osteoblasts form spongy bone

Young kids bones don't break that easily

Factors that led to this

Diet

Lactose intolerance

Bones are still going through growth stages

Junk food mainly consumed

Needs more nutrients

Fragment of bone in the wound

Breaking elbow (olecranon)

No consumption of dairy

Part of humerus is protruding through the skin

Fragment of bone in the wound

Classification of fracture

Right elbow (olecranon) broken

Open compound fracture

Classification of fracture

Closed simple fracture

Other possible types of breaks

epiphyseal break

Greenstick break

Other possible types of breaks

Greenstick fracture

Displaced fracture

Incomplete fracture

Incomplete fracture

Factors to consider during treatment process

Location of break

Boys diet

Age of boy

Needs Ca for bone remodeling

So the bones can store Ca for the body's processes

Possibly not getting enough amino acids for osteoid production

Making 1/3 of matrix

The epiphyseal plate is still active in males until about 21 yrs

Diet would benefit from change

Mico & macro nutrients are important for the healing process

By adding more minerals so they are properly stored

So the body can use it for bone remodeling

Humerus break

If an epiphyseal break

A method for growth continuation should be addressed

Olecranon break

In order to maintain bone symmetry on all side of body

If a greenstick fracture

The bones need to be re-brocken

He would possibly require steroids or growth hormone

To stimulate mitosis for growth

So its important to look at factors possible for affecting growth

Possible treatments

Other consequences of not taking care of the breakage

Bones can grow at an angle

& Placed in the proper position

Once the proper methods are exercised to place bone in place

A cast or traction could be placed around bone

So proper repair can take place