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HPLC (Basic comp. HPLC (column (5a. types (analytical (separation…
HPLC
Basic comp. HPLC
1.MP reservoir
glass bottled with lid, to carry mp to pump, air bubbles affect detector, essential filter n degas mp bf use
fx: pass mp thru column at high p n constant flow rate, characteristics: pulse free output, avoid large dead vol btwn pump n injctor, interior resistance to corrosion
type
flow rate deter by flow resistance of column, restriction btwn pump n outlet, xsuit for HPLC analysis
given flow of liq, p dev depend on flow resistance, ad 2 jenis
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displacement type pump
ad; large, pulse free output dis: limitent capacity, inconvenience
create gradient prog,alter nature @ polarity solvent, must homogenous
intro sample soln to column via sample loop, zero dead vol valve,ada manual n auto
5a. types
analytical
separation,stainless steel, high speed, min solvent consumption
guard
small,extend column life,saturator
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- data processing n recorder
Mode LC
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- ion supression n ion pair c