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Polycomb/H3K27me3 chromatin profiling from <1K - Coggle Diagram
Polycomb/H3K27me3 chromatin profiling from <1K
scientific project
define the scientific project (with Luciano)
scATAC-seq?
RNA/DNA methylation profiling?
targets
EZH2
find good Ab
qPCR loci?
Other polycombs/targets?
H3K27me3
methods
cut&tag
no purif as henikof?
X-cut&tag
cut&tag+LinAMP (LIANTI/THS-seq design papers for inspiration)
cut&tag+barcoding(CoBATCH)
ChIL
ChIPseq
TrueMicro H3K27/Polycombs: scale down
TrueMicro+LinAMP
model
Luciano's cell culture ( at Diagenode?)
knock-out
K562