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Fluorescent thermometer (Polymer based thermometers (Diffuse throughout…
Fluorescent thermometer
Polymer based thermometers
Incubation cell with thermometer containing medium
Or microinjection
High biocompatibility
Possible non-uniform response
Diffuse throughout the cell, lack of organelle -specific
Fluorescent proteins
Transfection
High biocompatibility
Laborious preparation procedure
Diffuse throughout the cell, lack of organelle -specific
Uchiyama et al.: each organelle has a different T within the cell
Small molecule organic dyes
Easily obtained
Stain easily b/c the small size
Good chemical stability
Organelle targeted
Long term may cause photobleaching
Incubation cell with dye containing medium
Introduction
method
Advantages
Localization
Disadvantages
Introduction
method
Advantages
Disadvantages
Localization
Most practicable
Practicable
Introduction
method
Advantages
Disadvantages
Localization
Least practicable