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BIO340 (Eukaryotes (Composition, Histone Protein, nonhistone protein,…

- - - - Rho-dep
      - Rho-indep
    - - sigma factor
      - RNA Pol Core enzyme
  - - - TATA Box
- - - - small ribosomal subunit - 30S
      - 3 IF (initiation factor proteins)
        
        IF2
        
        IF3
        
        helps binding between mRNA and 30S
        
        IF1
      - mRNA
        
        Shine-Dalgarno sequence
      - GTP (guanine triphosphate)
      - Large ribosomal subunit - 50S
      - initiator tRNA
    - - EF-Tu
        
        escort tRNA to A site of 30S subunit
      - EF-G
        
        (along with GTP) promotes translocation to 3' location
    - - Release factors - RF
        
        RF1, RF2
        
        recognize stop codon
        
        RF3
        
        Recycle RF1 and eRF1
        
        RRF
        
        Ribosome recycling
  - - - Elongation Factors - eEF
        
        eEF-1
        
        escort tRNA to A site
        
        eEF-2
        
        Promotes translocation
        
        like Bacterial EF-G
    - - Release Factors - eRF
        
        eRF1
        
        recognize stop codon
        
        eRF3
    - - initiation Factors - eIF
        
        eIF1