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Kv2.1/Kv8.2 (Preferred stoichiometry (IP method (Cloning (GFP-HA-FLAG-Kv2…
Kv2.1/Kv8.2
Preferred stoichiometry
IP method
Cloning
GFP-HA-FLAG-Kv2.1 :check:
Kv8.2-Myc-FLAG :check:
Co-IP
FRET
Cloning
Design primers
Ang cloning (restriction/ligation)
Expression stoichiometry
Constant Kv2.1
Constant Kv8.2
Retina
ddPCR (Shivangi)
IP from retina
Kv8.2 homotetramers
co-IP
GFP-HA-FLAG-KCNV2
BN-PAGE N=1
X-linking
Cloning (controls) :star:
T1 -> Artificial Tetramerization domain
T1 deletion