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探討Pseudaminic acid (Pse)在包氏不動桿菌之致病性和臨床重要性及其在台灣之分佈情形 (子計畫二 運用Pseudaminic…
探討Pseudaminic acid (Pse)在包氏不動桿菌之致病性和臨床重要性及其在台灣之分佈情形
子計畫一
以動物模式探討Pseudaminic acid (Pse)對於包氏不動桿菌感染之致病性
C. elegans
model
C. elegans
survival assays
C. elegans
phenotype change and genomic analysis
Biofilm formation and measurement
Mouse infection model
Lung, spleen, and bronchoalveolar lavage fluid bacterial burden counting
mRNA genes expression of cytokines in mice lung
ELISA assay for cytokines release in bronchoalveolar lavage fluid
Histological examination of lung tissue
Survival analysis
子計畫二
運用Pseudaminic acid (Pse)共軛寡醣分子所誘導的抗體,去偵測台灣醫院臨床包氏不動桿菌表面多 醣體含Pse的分佈
Extraction of surface polysaccharide of
A. baumannii
54149
Conjugations of Ab 54149 oligosaccharide with carrier protein
Production of antiserum against capsular polysaccharide-carrier protein conjugates
Extraction of capsular polysaccharide from clinical
A. baumannii
isolates
Dot-immunoassay
子計畫三
包氏不動桿菌表面多醣體所帶有之Pseudaminic acid (Pse)在包氏不動桿菌血流感染之臨床重要性
Data and bacteria collection
Antimicrobial susceptibility
Molecular typing by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST)
Biofilm formation measurement
Sequencing of genes associated with biosynthesis of Pse