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Week 6 Imaging and Resolution (resolution (sensor resolution (effective px…
Week 6 Imaging and Resolution
resolution
sensor resolution
aliasing
effective px size = native px size / magnification
decrease native
increase magnification
decrease S1
optical resolution
limitation
no real image
Point spread function/ airy disc
diffraction
solution
larger aperture
measurement
line-pair phantom
modulation transfer function
microscope
bright field
bright illumination
thin sample
require staining to increase contrast
simple,cheap
light transmit through the sample
dark field
image light scatter
contrast improved
sample is strongly illuminated
can lead to damage
fluorescence
principle
stokes shift
photon absorbed
electron exited
electron relax to low energy state
low energy light emitted
label
limitation
photobleaching
chemical damage
limit exposure time