The pGLO plasmid is a circular strand of DNA that contains a bla gene encoding for the resistance of the antibiotic, ampicillin(Amp), and is genetically engineered to contain a Green Fluorescent Protein operon regulated by the Arac regulator. The Arac protein as a repessor of the original arabinose operon is blocked when arabinose(Ara), a type of sugar, binds to it, leading to the expression of the GFP gene. (pGLO intext) In the experiment, two independent variables were tested with the pGLO plasmid and bacteria, the presence of pGLO and the environment that the bacteria was grown in. Through the results of growing the bacteria without pGLO plasmid in an agar plate with Luria Broth(LB), a bacteria food, Ara, and Amp, it was seen that the amp, as a selective pressure, killed the bacteria due to the lack of resistance that could only be expressed with the bla gene located in the pGLO plasmid. Similarly, there was no glowing phenotype as there were no bacteria for arabinose to activate the operon for.