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Genomic Library and Complimentary library (cDNA (Used for (making cDNA…
Genomic Library and Complimentary library
gDNA
genomic DNA of an organism
Used for
Genomic library
Formed by
Contain whole genomic fragments (Exons, introns, promoters, origins of replication)
Form genomic library's by being cut into cloneable pieces as randomly as possible using restriction endonucleases
Used for
Screening for genes of interested
using radioactivity or antibody for colony hybridisation
cDNA
Complimentary DNA
Used for
making cDNA libraries
Formed by reverse transcriptase making single strand of cDNA
RNase H degrading mRNA molecule
DNA polymerase ebsolon forming complimentary strand and ligase joining the fragments
makes double stranded cDNA
Produced by
Reverse transcriptase dNTPs
Techniques
Sothern Blotting