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What are the main methods to make GM crops and why are these controversial…
What are the main methods to make GM crops and why are these controversial. What are the genome-editing techniques/ next generation techniques and how do they address these concerns?
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Gemone-editing techs
ZFN
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the recognsie specific nucloties int he flanking regoins anddimarse at the site creating a cut. there is no contorl over this so need to sceeen ,
3-6 zinc finger repoates. zinc finger arrays bind to the opposite strands of DNA causing the nuclaease to dimasre and cleave the DNA.
can be introduced inot the cell as plamids in protoplasts, as prified protein or adeno-accidted virus.
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Others
Mutation breeding
Site oligonulitide directed mutatenissi, where one nultided is altered fro a diseared trairt. can create a nutlaitoe with flanks that muatch the target site and nutltieded swtich.
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