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2.2.2 - tracing the development and movement of the products of…
2.2.2 - tracing the development and movement of the products of photosynthesis
near-infrared imaging
non destructive
study movement of water
shorterwavelength
plants reflect infrared
study carbohydrate content in leaves
glucose
photosynthesis
fluorescence imaging
measure movement of water
rate of photosynthesis
genetic impact
detected by: example
non-destructive
shining blue light on plant -> cause plants to glow
magnetic resonance imaging (MRI)
strong magnetic field and radiowaves
3D imaging of root structures
application: grow plants in clear containers so roots can be studied by MRI
no stress to plant
study structure, metabolism and phycological properties
structure -> root system
physiological properties -> a property having to do with the functioning
image can combine wiht images from PET or NT
greater detail and functional information about transport and process
involve detection of radiation produced by radioisotope
PET: positron emission tomography
NT: Neutron tomography
X-ray computed microtomography
non-destructive
internal structure of plants
sample positioned in x-ray beam is rotated and hundreds of images from different angles recorded
any angle observed and spatial arrangement of internal tissues studied
images analysed and reconstructed into 3D computer-generated image
photosynthesis process
radioactive tracer -> carbon-14
sunlights acts on chlorophyll to begin manufacturing organic compounds
isotopes
different forms of the same element
radio isotopes: emits radiation (radioactive)
emission of radioactive articles/waves measured by many methods
useful tracers
determined that water was source of oxygen gas released in photosynthesis
pathway of glucose produced in photosynthesis, can be traced
computer converts information into 3D images
see where radioactivity labelled chemicals are moving or stored in living organism
RRIS
real-time radioactive imaging system
non-destrucitve
visualises movement of substances in phloem
PlanTIS
PET scanner for plants
traces radioisotope carbon-11 -> glucoseolecule
3D imaging
digital photos taken at various points and angles
photos are up together in a computer program to from 3D image
measurements made and external structure studied using image
visible light
non-destructive
qualitative results
leaf health
quantitative results
leaf size
far-infrared imaging
non-destructive and can be, don't on sight
measures temperature
gradation of red colour
longer wave length
plants reflect infrared
helps track salinity of plant and ability to cope
salt levels
see whats in the soil
leaf temperature is increased when salinity is increased