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MitoPQ in vivo (Technical (Blended established techniques (PRDX3, BN-PAGE,…
MitoPQ in vivo
Technical
Catalyst free click chemistry
Co-IP
Easier workflow
Blended established techniques
PRDX3
BN-PAGE
LC/MS/MS
Unbiased
Ability to identify hydrophobic sub-units
MitoPQ
Selective
Theory
Redox signalling
generator specific
local loops
concept of auto-regulation
Complex I
test of flavin pop-out
test of reach to Q and P modules
Development
completely novel
Limitations
Older not tissue specific
Difficulty normalising
Fully quantifying
Outcomes
New targets
Adds to a new target
In a better journal if identify a new target
why read it
compound readership
wider readership