impedance chip experiment (material & methods (methods (첫번째 실험 (실험의…
impedance chip experiment
material & methods
Nacl = [0.65, 1.25, 2.5, 5, 10]mg/ml
CT = [2.5, 5, 10, 25, 50] ng/ul
Impedance measuring instrument
We used a USB ocilloscope with an array of interface boards (multiplexer and amplifier circuit) (Analog Discovery 2) to reduce the amount of space required for impedance measurements and ease control.
Impedance was measured by connecting the chip connector and the interface board.
micro pcr device
micro pcr chip
We have developed a PCB based PCR chip.It has based structure that a heater pattern and a thermistor at the bottom, and a copper pattern for heat spreading on the top. and a thin polypropylene tape above the heat spreader separates the reagent and the PCB substrate. (REFER)
gold surface PCB chip : Due to the nature of the gold, the chemical reaction of the reagent is low and it has been shown that it is a good material as hydrophilic in the previous paper.[REFER] In this experiment, the heat spreader was covered with gold. The gold surface of the heater spread size is cut at intervals of 0.1 mm to serve as a pair of electrodes.
polypropylene tape : Make a 2 mm hole around the center of the pair of electrodes on the tape.The reagent injected into the chamber was detected and a hole was drilled to measure the impedance.
We reported a real-time miniaturized PCR system that detects fluorescence using a camera.[REFER]
실험의 목적: 미소논문 REFER 확인 실험[주파수 의존성 실험 ?은 어찌할까]
염두해야할 미소 중요 결과
일주일간 cv는 최대 3.2% 였으며, 10mg/ml가 가장 컸다.
일간 변동이 있었으며 농도가 클수록 큰 변동을 보인 것을 알 수 있다.
연속적으로 측정한 CV와 일간 평균 임피던스의 변동과는 관계가 없어 보인다.
Nacl 실험 후 CT실험
10일간 하루에 각 농도별 10번씩 측정
농도별 cv값, 농도별 평균 imedance 구하기
실험의 목적:PCR 증폭 과정의 시작부터 끝까지 측정되어 각 증폭주기의 임피던스 변화뿐만 아니라 한주기의 각 증폭 단계의 변화를 추적
36ul 양의 시약을 넣고 실험
This paper presents a real-time PCR system capable of DNA quantitation through impedance measurement.
We reported a real-time PCR system which has a printed circuit board (PCB) based PCR chip and detects fluorescence using a camera. In this system, the PCR chip is based on a PCB with a heater pattern and a thermistor at the bottom, and a copper pattern for heat spreading on the top. A thin polypropylene tape above the heat spreader separates the reagent and the PCB substrate. From this chip, an electrode is constructed by cutting the heat spreader in half. The electrode is exposed to the reagent through a hole in the polypropylene tape. Impedance is measured from the beginning to the end of the PCR amplification process to track not only the impedance change in each amplification cycle but also the change in each amplification step in one cycle. The frequency dependency and impedance detection methods are also investigated
This research will present the possibility of label-free real-time PCR system using impedance detection.