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Photosynthesis (Light dependant reactions (Consequence of proton gradient…
Photosynthesis
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Reaction centre
the pair of chlorophyll a molecules are converted to a high energy state. As a result the redox potentials of the activated pigments are reduced. ( Kicks an electron from a low energy state to a high energy state)
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Redox carriers
Quinol reduced on one side of the membrane then oxidised- picks up proton as this happens and creates a proton gradient.
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Electrons come from photosystem 1
these are carried to Photosystem 2, where they are converted to energy
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Hill Reagents
These are substances that accept electrons transferred from water by the photosynthetic light reactions and thus lead to evolution of oxygen up illumination.
The Hill reagent is NADP+, but some other chemicals can substitute for it in vitro
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Ribulose biphosphate carboxylase/ oxygenase: The most abundant protein on earth + Slowest enzyme that we know of. Half of the nitrogen in our body comes from Nitrogen in Haber bosch process
16 sub unit protein composed of 8 large sub units coded by chloroplast DNA and 8 small sub units coded by Nuclear DNA
DCPIP - when this is added it can strip chloroplasts without NADP+, the reagent was decolourised and the chloroplasts began to produce oxygen even in the absence of carbon dioxide.
Chloroplast F0 domains have up to 15C sub units. F0 moves things around one click - allowing about 14 protons, giving 3 ATP
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Oxygen is a waste product of photosynthesis. Iron Formation is product of increased oxygen in the atmosphere
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