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Microbial growth (Environmental Factors Affecting Growth (Osmotic Effects…

- - - - Mesophile: midrange temperature
      - Thermophile: high temperature
      - Psychrophile: low temperature
      - Hyperthermophile: very high temperature
- - - - living
      - reproducing population
    - - Spread-plate method
      - Pour-plate method
  - - - Cannot distinguish between live and dead cells without special stains
      - Small cells can be overlooked
      - Precision is difficult to achieve
      - Phase-contrast microscope required if a stain is not used
      - Cell suspensions of low density (<106 cells/ml) hard to count