UNIT 2: Two-way WS and Split-Plot (BSxWS) designs

Two-way WS design

Split-Plot design (Mixed design)

two WS factors are crossed

3 effects of interest:
-main effect of A,
-main effect of B
-interaction effect (AxB)

Interaction tested first

Significant

Non-significant

test the simple effects

A per level of B

B for level of A

Test the main effects (no need to remove the interaction term from the model)

lab experiments

The Univariate Method

The Multivariate Method

Two-way WS design --> Three-way BS design:
-fixed factors: A&B,
-random factor: person

Every fixed effect must be tested against its interaction with the random effect (for any factorial design with one random factor and other factors fixed)

Two-way BS:


-main effect of A -> F= MS(A) / MS(A x person)
-main effect of B -> F= MS(B) / MS(B x person)
-interaction -> F= MS(A x B) / MS(A x B x person)

if n=1 per cell -> interaction cannot be seperated from error -> both pooled into MS(resid) -> MS(resid): the denominator for testing the interaction

2 x 2 WS design

2*k WS designs with k>2 levels

transforming original repeated measures into
a) their average
b) orthogonal contrasts
(contrasts -> weighted sum of the repeated measures)

cross products of two columns also zero

contrast coefficients add up to zero per column

Normalization (dividing each coefficient with the square root of SS (sum of products) -> coefficients of +.05 and -.05

computing a weighted sum of the repeated measures for each person

testing H0: μ = 0 , for that new variable with the one sample t-test

testing if the mean of the new compared variable (with contrasts) differs from 0

paired t-test (one sample but with measures at 2 different time points

tablo 1

exp: W = (+1Y11) + (-1Y12) + (-1Y21) + (+1Y22)

Does W = 0 ?

tests the interaction effect (1,-1,-1,1)

interaction effect represented by the products of each of these k-1 contrasts with the only contrast for the factor with 2 levels

the factor with k levels will be represented by k-1 orthogonal contrasts (df=k-1 for its main effect)

example with k=3, using polynomial contrasts

each column total=0

cross product for pair of column=0

tablo 2

Inclusion of a BS factor into a WS experiment (gender & gender by condition effects)

BS experiment with repeated outcome measures (after treatment, follow-up)

Prob1: sample size needed for BS effects much > than sample size needed or WS effects --> the power for BS effects in WS experiments is usually low

Prob2: If the BS factor is not experimentally controlled but a person characteristic (exp. gender) --> all BS and BS*WS effects suffer from the same confounding problem as non-randomized studies

most important application of the split-plot desing

The Univariate Method

The Multivariate Method

assumes sphericity for the WS effect and the WS*BS interaction effect (which is by definition satisfied if the WS factor has 2 levels)

Obtained by part of the multivariate method with SPSS GLM

not easily applicable with SPSS because the random factor person is nested within the BS factor (each person is a member of one group only)

more than 2 groups (k>2 levels for the BS factor)

2*2 design with 2 groups and 2 measures per person

more than 2 repeated measures (k>2 levels for the WS factor)

k-1 instead of only one contrast

exp: linear and quadratic contrasts if k=3

same as 2*2 design but we can add pairwise comparisons for the BS factor

measures transformed into new variables
-average
-contrasts

Average (A) -> DV for testing the BS effect

Analysis: One-way BS ANOVA (unpaired t-test if there are only 2 groups)

Difference (D) -> DV for testing the WS and WS*BS effects

The Model Equation

Yij = β0 + β1Xij + eij = μ + αj + eij

Yij = A for testing the BS effect

Yij = dv for person i in group j

Yij = D for testing the WS and WS*BS effects

β0 + β1Xij + eij = a regression model with treatment x centered (X=-0.5 for control, X=+0.5 for treatment)

β0 = grand mean

β1 = group difference of interest with respect to the Y (either A or D)

μ + αj + eij = the traditional ANOVA model

μ = grand mean

αj = deviation of group j from the grand mean