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DNA PROFILING (key terms (INTRONS (A non-coding sequence of DNA), VNTRs…
DNA PROFILING
Mini and Microsatellite
Minisatellite
20-50 base pairs
Repeated up to 100 times at the same
position
within
introns
Microsatellite
Smaller repeats
key terms
INTRONS
A
non-coding
sequence of DNA
VNTRs
Variable number tandem repeats
STRs
Short tandem repeats
restriction site
Region
of DNA with a
Particular base sequence
Process in which image is produced of the
pattern
of satellite DNA
VNTR and STRs can be used to discriminate between unrelated people
The
pattern
is
unique
for
each person
Different
number
of
repeats
are
inherited
from each
parent
RESTRICTION ENZYMES
Break down large DNA molecules into
smaller pieces
called
DNA fragments
Cut a
DNA
molecule at specific
restriction sites
Sequence creates a
specific 3D
shape which is
complementary
to the enzymes
active site
Hydrolyses
cuts the
phosphodiester
bonds on
each strand
of the
doable helix
In the
same place
The
sugar
r
back bone
is
broken