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The Cell (Cell Tour (Microscopy (Light Microscopy (Fluorescence: locations…
The Cell
Cell Tour
Microscopy
Light Microscopy
Fluorescence: locations of specific molecules in cell can be revealed by labeling the molecules with fluorescent dyes or antibodies
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Phase-contrast: variations in density within the specimen are amplified to enhance contrast in unstained cells
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Scanning Electron Microscopy: useful for detailed study of topography; scans the surface that is usually covered with thin film of gold
Transmission Electron Microscopy: used to study the internal structure of cells; specimen has been stained with atoms of heavy metal
Cell Fractionation: used for studying the cell being able to separate cell components based on size and density
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Active Transport: the pumping of solute across a membrane against its gradient which requires work; the cell must expand energy
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Diffusion Relationships: Osmosis and tonicity are larger water molecules that move across the membrane by simple diffusion