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10/26, OPTiSPIM (第五段 (OPTiSPIM (four main modules (I. SPIM_illumination …

- - - - recording nonfluorescent features
  - - - recording large samples at high-resolution
  - - - I. SPIM_illumination
        II. Transmission illumination
        III. sample positioning
        IV. detection
        
        II. tansmission illumination optics:
        Spatially and temporally incoherent light illuminates and
        is imaged on the camera as shadow pattern.
        <designed DOF covers half the width of the sample>
        
        use an iris placed behined the detection objective to reduce the
        NA of the detection and thereby control the DOF
        
        III. Sample position
        the sample is held in place, ''translated(平移), and "rotated".
        Traslation: 3 axis
        Rotation: vertical axis
        
        IV. Detection module
        The detection module is shared by both techniques.
        4f telescope and filters
        
        OPT fluorescence mode:
        fluorescence lamp is coupled into the detection path
        
        1 more item...
- - - - modes
        
        I. fluorescence (emission) OPT
        II. bright field (tranmission) OPT
        
        limitation FOV ,
        high resolution
- - - - lateral resolution:
        SPIM 3 micro
        OPT 4 micro
        但是兩個都可以分辨內皮小靜脈