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Genes and the Genetic Basis of Metabolism and Development (Analysis of…
Genes and the Genetic Basis of Metabolism and Development
Analysis of Gene and Recombinant DNA Techniques
Identifying DNA Fragments
plastid DNA is a small molecule containing only 60,000 to 80,000 base pairs. Treatment with the Pst I restriction endonuclease produces about 10 to 20 fragments.
DNA Cloning
method of placing DNA fragments into bacteria
Restriction Endonucleases
DNA Sequencing
DNA to be sequenced is first cloned to obtain a large sample and is then divided into four batches
Nucleic Acid Hybridization
single-stranded nucleic acids (DNA or RNA) are allowed to interact so that complexes called hybrids are formed by molecules with similar, complementary sequences.
Sequencing Entire Genomes
effective for fragments less than several hundred bases long
Storing Genetic Information
The Genetic Code
The Structure of Genes
Protecting the Genes
Transcription of Genes
Viruses
Virus Metabolism
invade a living cell in order to reproduce
Formation of New Virus Particles
Origin of Viruses
portions of genes of the host species or a species closely related to the host
Plant Diseases Caused by Viruses
Virus Structure
contain only protein and nucleic acid.
Genetic Engineering of Plants
Protein Synthesis
Ribosomes
small particles that “read” the genetic message in mRNA
tRNA
During protein synthesis, amino acids are carried to ribosomes by ribonucleic acids
mRNA Translation
synthesis of a protein molecule by ribosomes under the guidance of mRNA