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MICROBIAL DIVERSITY(PROTIST AND FUNGI) (FUNGI (major fungal groups…
MICROBIAL DIVERSITY(PROTIST AND FUNGI)
PROTIST
general charateristics
Most are unicellular
chemoorganotrophic
role in recycling nitrogen and phosphorus
Plasma membrane
structure similar to multicellular plants/ animals
Cytoplasm
Outer gelatinous ectoplasm
Inner fluid region termed endoplasm
Pellicle
provides support
supergroup
super group Excavata
Fornicata
Microaerophilic
epidemic diarrhea from contaminated water
flagellated and lack mitochondria
harmless symbionts
exceptions
Giardia – causes diarrhea
Hexamida salmonis – fish parasite
H. meleagridis – turkey pathogen
Parabasilia
Most are flagellated
Lack a distinct cytosome
phagocytosis to engulf food
Two subgroups
Trichonymphida
obligate mutuals of wood-eating insects
such as termites
release cellulose for digesting
ecdysone hormone produced by host triggers sexual reproduction
Trichomonadida
do not required oxygen
possess hydrogenosomes
asexual reproduction
symbionts of digestive, reproductive, and respiratory traits
Euglenozoa
found in fresh water
1/3 photoautotrophic
representative phototroph
Super-Group Amoebozoa
Amoeboid motility
Naked amoebae
surrounded only by a plasma membrane
Testate amoebae
plasma membrane covered by material made by amoeba or obtained from the environment
Supergroup Rhizaria
general characteristisc
Amoeboid in morphology
distinguished by fine pseudopodia
axopodia
• filopodia supported by microtubules • protrude from the axoplast • primarily used in feeding
Stramenopila
diatoms
marine plankton
produce 40% to 50% of organic carbon in ocea
golden and brown algae
öomycetes and labyrinthulids
Chlorophylls and accessory pigments
Frustule – two-piece cell wall of silica
Alveolata
Apicomplexans
Parasitic
Plasmodium, the cause of malaria
Dinoflagellates
marine plankton
cause phosphorescence/toxic blooms in seawater
Nutritionally complex
Trichocysts are defensive proteins
Symbiotic forms (zooxanthellae)
Ciliophora
Chemoorganotrophic
Found in both benthic and planktonic communities
many cilia as locomotory and feeding organelles
Reproduction
sexual by conjugation
asexual by transverse binary fission
Supergroup Archaeplastida
containing a photosynthetic plastid
arose through an ancient endosymbiosis with a cyanobacterium
FUNGI
True Fungi: Eumycota
general characteristics
Chemoorganoheterotrophs with absorptive metabolism
Lack chlorophyll
Saprophytes
absorb nutrients from dead organic
Reproduce sexually and asexually
Fungal Distribution and Importance
Primarily terrestrial
form associations
– mycorrhizae – associations with plant roots
– lichens - associations with algae or cyanobacteria
Decomposers
Industrial importance
fermentation – yeast used in making bread, wine, beer, cheese, soy sauce
organic acids – citric and gallic acid
antibiotics – penicillin, griseofulvin
immunosuppressive agents – cyclosporin
Research use
geneticists, cytologists, biochemists, biophysicists, and microbiologists
Saccharomyces cerevisiae • yeast model system for cell biology, genetics, and cancer
major fungal groups
Basidiomycetes (club fungi)
examples include rusts, shelf fungi, puffballs, toadstools, mushrooms
sexual reproduction form basidium
Impact on Human
Decomposers
Edible and non-edible mushrooms
Pathogens of humans, other animals, and plants
e.g. Cryptococcus neoformans
Urediniomycetes and Ustilaginomycete
Ascomycetes or sac fungi
Genus Aspergillus
A. fumigatus
– ubiquitous environmental – allergies and significant pathoge
A. oryzae
– production of fermented foods – important in biotechnology
Aspergillus – 37 Mb genome, model system
general characteristics
some used as research tools
some yeasts and truffles are edible
some are human and plant pathogens
general characteristics
Pathogenic Ascomycota
Claviceps purpura
on higher plants
• toxic condition from eating infected grain
• due to lysergic acid diethylamide (LSD
Candida, Blastomyces, Histoplasma
human pathogens
Aspergillus – aflatoxins and cancer
Glomeromycota
Aseptate flat hyphae
to penetrate host plants; produce large, multinucleate spores and only reproduce asexually
importance as mycorrhizal symbionts of vascular plants
intracellular associations within roots
mutualistic relationship
,delivers soil nutrients to the plant; plant provides carbohydrates to fungus
fungus helps protect host from stress
Zygomycota
Most are saprophytes
Form coenocytic hyphae containing numerous haploid nuclei
industrial importance
foods, antibiotics and other drugs, meat tenderizer, and food coloring
reproduce asexually by spores
Sexual reproduction occurs when environmental conditions are not favorable
hormone production causes hyphae to produce gametes
requires compatible opposite mating types
gametes fuse, forming a zygote
zygote becomes zygospore
Genus Rhizopus
R. stolonifer
grows on surface of moist carbohydrate rich foods such as bread
hyphae quickly cover surface as rhizoids, absorb nutrients
stolon hyphae become form new rhizoids
Importance
Rhizopus-Burkholderia symbiosis
seedling blight in rice bacterium Burkholderia growing within Rhizopus produces toxin
Used to produce tempeh from soybeans
Used with soybeans to make sufu curd
Commercially
used to produce anesthetics, birth control, alcohols, meat tenderizers, yellow coloring in margarine
Chytridiomycota
also called chytrids
Produce a zoospore with single, posterior, whiplash flagellum
Asexual and sexual reproduction
degrade cellulose and keratin
Microsporidia
Obligate intracellular fungal parasites
Aquatic birds are common hosts and contribute to large numbers of spores in environmen
Transitional form is a spore structure
capable of surviving outside the host
Structurally similar to ‘classic’ fungi
contain chitin, trehalose, and mitosomes
morphology is polar tube essential for host invasion
lack mitochondria, peroxisomes and centrioles
Pathogenesis
Human infections – Enterocystozoon bieneusi • diarrhea • Pneumonia
– Encephalitozoon cuniculi • encephalitis • Nephritis