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Machaniari (Key Points (can be applied to all tissues, Donor factors,…
Machaniari
Key Points
can be applied to all tissues
Donor factors
decellularisation cycles
threshold DNA values
Structural integrity
Cell types for reseeding
Biological clues
Viability post transplant
Advantages
Vasculature retained
Matrix
Existing procedures
Biologically compatible
Preimplant
Confirm chrondrocytes
Cyctokeratins 5 and 8
DAPI To detect nuclear DNA
Disadvantages
Cell density
long term viability
Continuous remodelling in vivo
Cells
Bone marrow
Trachea
Trachea from donor
stained for DNA
Absence of MHC antigens
Novel Bio Reactor
2 different cultures
Macchiarini (2008)