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Selective plane illumination microscopy techniques in developmental…

- - - - thickness
      - imaging modality
- - - - confocal
        
        100 micrometer
    - - fluorescence is excited only where it is needed, which leaves the remainder of the sample unexposed and therefore free of photo-bleaching and -damage.
        
        multi-photon microscopy
        
        in fluorescence light-sheet microscopy
        
        In light-sheet microscopy, however, a different approach is taken to selectively illuminate the area of interest while at the same time preventing the sample from damage, as discussed in the following section.
        
        Light Sheet Fluorescence Microscopy
        
        The idea behind SPIM and other light-sheet-based microscopy techniques is to illuminate the sample from the side in a well-defined volume around the focal plane of the detection optics
        光片顯微鏡技術，產生片狀照明光從側面打入，只激發焦平面上的激發螢光。
        
        the common general principles
        
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        there are many different implementations of this idea
        
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