Please enable JavaScript.
Coggle requires JavaScript to display documents.
Recombinant antibodies / Therapeutic proteins (Example steps in rAB…
Recombinant antibodies / Therapeutic proteins
Antibodies
mainly b-sheet domains
in natura systems - produced polyclonal mixtures
the aim is to have monoclonal Abs
Recombinant antibodies
ScFv (single chain fragment variable)
sdAb (single domain)
Fab
ADVANTAGES
High affinity
wide target compound distribution
Selection conditions can be adjusted to faveor deired tupe of binders
Antibody genes available for further manipulation
IV method
Only small amounts of antigen needed
Antibody generation is rapid
Human antibodies
Bypassews immunization
Reproducible
Antibody libraries
Require display platforms
Library definitions
Synthetic rAB library
Framewor regions maintain 3D structure
approx 80% of V-domains AA sequence
Incorporation of unique and general restriction sites
high diversity
rapid randomizations of individual CDR loops
modularity
CDR and framework shuffling
Optimization for expression
Panning (kasvatus ja rikastus, slideissa hyvä kuva)
Expression in E. coli
Example steps in rAB production
Cloni
Selection of antibodies from the library by phage display technique
Choosin an appropriate rAB library