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Protein characterization (Scattering methods (Combining different methods,…

- - - - Reducing agent (DTT might be present)
    - - Take into account also
        
        Intrinsic charge
        
        disulphides
        
        post-translational modifications
  - - - separates by molecular weight and intrinsic charge
      - efficient in chemical conjugation state recognition
      - efficient in post-trans recognition
      - oligomeric state preserved
- - - - is related to hydrodynamic radius of the particle
      - suitable for
        
        0.3nm - 10um
      - can be used in QC
  - - - Challenge
        
        1 dimensional data into 3 dimensional model
  - - - 90 degree angle
        
        yields particle size
    - - viruses and other large particles
        
        multi-angle LS
        
        yields size without known concentration
  - - - protein consentration
    - - molecular weight
    - - separation of molecules according to molecular size
    - - hydrodynamic radius
- - - - Indirectly can obtain
        
        Entropy
        
        stoichiometry
  - - - SLS, DLS
    - - NMR
      - Fluorescence spectroscopy
      - UV/Vis
      - Fluorecence
    - - Radioactive immunoassay
  - - - Measures the amount of immobilized material on sensor
        
        Reveals binding and dissosiation kinetics
        
        Typical setup
        
        Studying the biding
- - - - Avoiding operating in Isoelectric point is useful