Week 3: Immunohistochemistry: Preserving and Studying Cells of the Brain (…
Week 3: Immunohistochemistry: Preserving and Studying Cells of the Brain
ONE: Understand the basics of specimen preparation for histology and immunostaining techniques
Fixation of tissue, processing and begging tissue, mounting tissue on slides.
Basic Histological staining techniques
Why examine tissue and cells?
Because it allows us to study tissue anatomy and cytoarchitecture
Distribution of proteins
Pathological changes associated with disease
It is essential in (A) clinical diagnostic neuropathology and (B) basic and translational neuroscience research
Tissue Preparation for histology
1) To preserve the tissue in as life like a manner as possible. 2) to prevent irreversible cell/tissue destruction.
Common methods of tissue preservation:
chemical fixation and crypopreservation
Chemical Fixation Model:
Chemical fixatives stabilise proteins and other macromolecules
Chemical Fixation Process:
TWO: Explain, with examples, how different biological stains can be used to study disease pathology in the central nervous system
THREE: Understand the basic principles of monoclonal and polyclonal antibody production
FOUR: Describe the different methods used to visualise antigen-antibody complexes
SIX: Critically evaluate the published research that has employed these techniques
FIVE: Explain how immunostaining techniques can be used to study the presence and location of specific proteins in a cell