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Elizabethkingia species** (2007重新分類 (E.menigoseptica, E.miricola, E…
Elizabethkingia
species**
2007重新分類
E.menigoseptica
E.miricola
E.aonphelis
Molecular typing
RAPD+Capilary Electrophoresis system
Antimicrobial resistance
Quinolone resistance
Alterations in target enzymes-QRDR
Levofloxacin / Ciprofloxacin
phenomenon
Recent reference
QRDR,gyrA ParC
antimicrobial susceptibilty
susceptible
resistant
intermediate
E.spp
DNA gyrase, subunit A,(type II topoisomerase)
6 more items...
ParC(DNA topoisomerase IV subunit A)
2 more items...
ParE DNA topoisomerase subunit B
1 more item...
Plasmid-mediated quinolone resistance
Altered drug permeation-PUMP
Antimicrobial resistance
Quinolone resistance
Altered drug permeation-PUMP
Plasmid-mediated quinolone resistance
賣點:目前E.spp未有相關文獻討論
Alterations in target enzymes-QRDR
Levofloxacin / Ciprofloxacin
phenomenon
E.spp
ParE DNA topoisomerase subunit B
ParE(DNA topoisomerase IV subunit B)
we found one change in the amino acid sequence of GyrB of strain 197 (Ser-494 to Thr), and a Gln substitution was observed in ParE Pro-424 of strain 112.
Novel mutations were found at five different codons and comprised Glu453-Gly His461-Tyr), Ala498-Thr , Val512-Gly, and Ser518-Cys.
ParC(DNA topoisomerase IV subunit A)
初步發現,其ParC gene no mutation in QRDR region such as AA site 78 80 84
#
合成Primer(已完成),共有二組。一組for E.anohpelis and e.miricola,一組for E.menigoseptica 找不到更conserved sequence
DNA gyrase, subunit A,(type II topoisomerase)
gyrB
DNA gyrase, subunit B (type II topoisomerase)
AA Asp426Asn Lys447Glu
這些抗藥菌株基因分型的結果
合成Primer(已完成)
Result analysis<待基龍米克斯定序結果分析>
PCR/Sequcing<請煜瑄完成後續>
Mismatch Amplification Mutation Assays (MAMA) for Rapid Detection of gyrA and parC Mutations
這些抗藥菌株基因分型的結果
合成Primer(已完成)
Result analysis<待基龍米克斯定序結果分析>
PCR/Sequcing<請煜瑄完成後續>
antimicrobial susceptibilty
susceptible
resistant
intermediate
Recent reference
QRDR,gyrA ParC
Known carbapenme resistance gene
ESBL
GOB,BlaB<已建立方法篩選>
CME<已建立方法篩選>
Molecular typing
RAPD+Capilary Electrophoresis system