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28373564 (Introduction (the role of PolD and PolE (14: PolD to PolE…
28373564
Abstract
Introduction
read 12, 13
14 PolE/PolD
exchange
Switching PolD/PolE
19: MCM structure paper
20: MCM Pumpjack motion
22: Cryo EM and FRET
how to trap CMG complex for EM
23: Johannes Cell
24, 25:PolA with CMG
PolE: heterotetramer
14, 24-36: coordination by CMG and PolE
the role of PolD and PolE
11, 14: PolD supports leading strand
14: PolD to PolE switching on leading
L-tag and PolD needs specific interaction
15: MCM N-terminal domain
processive collar
Results
Fig.1
DNA unwinding assay
good reference figure for Cryo EM
Fig.2
WT PolE: two lobes
PolE: 381.5 kDa
PolE: 4 sub units
42: CMG helicase O'Donnell
24: PolE CMG interaction
Fig.3
CMG:PolE=1:1dic
ATPase tier bonds to CMG
Fig.5
Dbp3 rotation
Discussion
14: polymerase switching event
PolA to PolD
PolD to PolE
L-tag needs PolD
PolD: stayed
related to DNA binding and release?
35: helicase processivity factor
fork stabilization factor
Mrc1
Tof1
Csm3
proved by yeast system