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Etroplus maculatus (experiments read (Muscle water content (MWC) (muscles…

- - - - Fish were anesthetized with clove oil
      - The gill filaments were dissected out, blotted dry and placed in ice-cold SEI buffer (sucrose 250 mM, EDTA Na2, imidazole 100 Mm and phenyl methyl sulfonyl fluoride (PMSF) 1 mM, pH 7.6) containing proteinase inhibitors (10 mg antipain,5 mg leupeptin and 5 ml aprotinin; v/v 100: 1)
      - immediately frozen in liquid nitrogen.
      - Homogenization was performed in 2-ml tubes with a POLYTRON PT1200E and 20 % (wt:vol)
      - homogenates were then centrifuged at 13,000 g and 4 C for 20 min. The supernatants
      - supernatant used for protein concentration estimated by Bradford using bovine serum albumin
      - and subsequent immunoblotting and enzyme activity
  - - - increased ventilatory movements of operculum, jerking movements and
      - frequent pulping of air at the surface of the medium
    - - Hemorrhages occur in the fins, which show many signs of
      - degeneration and shedding, such as splitting and breaking off of the fins at
      - their distal extremities.
  - - - stains
        
        haematoxylin-eosin
        
        for tissue morphology and cell integrity
        
        ulceration or inflammation
        
        Periodic Acid Schiff - AB-PAS
        
        number of mucus cells
        
        acidic mucus blue and neutral mucus magenta
        
        Alcian Blue
        
        SEM
        
        TEM
- - - - has ALPA,BETA , GAMA subunits
      - maintains intracellular homeostasis,
      - provides a driving force for
        many other ion-transporting systems
      - The NKA and NKCC play
        major roles in switching these systems efficiently to
        adapt in FW and SW