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Control of Microbial Growth, Growyh Control by Heat (Heat sterilization…
Control of Microbial Growth
Growyh Control by Heat
Heat sterilization widely used in controlling microbial growth
High temperature denatured macromolecules
decimal reduction time-
time required to reduce viability tenfold
Produce resistance cell called
endospore
- rapidly kill vegetative cell
autoclave
- a steam under pressure
Temperature can above 100
not pressure kill the microbe but high temperature
Radiation
Other physical control method
UV has sufficient energy to modification and break DNA; DNA also use in decontaminate surface
Ionizing radiation
; generate electron, hydroxyl radicle, hydride radical
Radiation is used in medical field and food industry
Chemical Control of Microbial Growth
Antimicrobial agent
can be classified; bacteriostatic, bacteriocidal, bacteriolytic
commercial and industrial
growth of human pathogen in external surface
Minimum inhibitory concentration-
smallest amount of agent needed to inhibit
Disc diffusion assay
uses solid media
Filtration
Depth filters; Hepa filter
Membrane filter; function more like sieve (nucleopore)