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Lec 10 (Nutrition,Culture and Metabolism of Microorganism) - Part 2,…
Lec 10 (Nutrition,Culture and Metabolism of Microorganism) - Part 2
Respiration: electron carrier
Respiration
Aerobic respiration
; where Oxygen as terminal electron acceptor
Electron transport system
NADH dehydrogenase
Flavoprotein (contains flavin prosthethic group); FMN,FAD
Cytochrome
Protein that contains heme prothethic gp
Accept and donate a single electron
Iron-sulfur protein; ferredoxin (carry electron)
Quinones
Hydrophobic non protein containing molecule
accept electron and proton but pass along electron only
Glycolysis
(common pathway catabolism of glucose)
Fermentation
Fermented substance is
both
e donor and e acceptor
Substrate-level phosphorylation (energy rich intermediate)
Anaerobic process
Yeast
: glucsose --> 2ethanol + 2CO2
Lactic acid bacteria
: glucose --> 2lactate
2 ATP produced
Glucose is consumed
Fermentative Diversity and the Respiratory Option
Fermentation classified by products
formed
3) Propionic acid
4) Butyric acid
2) Lactic acid
5) Mixed acid
1) Ethanol
6) Butanol
Fermentation classified by substrate fermented
2) Purine and pyrimidine
3) Aromatic compound
1) Usually not glucose
4) Amino acid
Saccharomyces cerevisiae-
can carry out fermentation and respiration
respiration generate more ATP
Fermentation occurs when anoxic
Respiration: proton motive force
Complex1 NADH donate electron to FAD while FADH donate to quinones