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Mounting,labelling,artifact (Procedures Mounting (drop of mounting medium…
Mounting,labelling,artifact
Procedures Mounting
- drop of mounting medium (permount) put on section and cover slip is mounted over the section
- as solvent of mounting medium evaporate, mounting medium harden and encloses the tissue sections in an almost solid subs and attaches the handled without damage to the tissues section
- suitable mounting medium dry quickly, has the same refractive index as glass (allow light to pass at the same rate as glass) and will not fade the staining of tissue
- after mounting medium harden, excess mounting medium from slide and cover slip is removed by razor blade and a Kim wipe moistened with xylene
- the pertinent identification info is recorded on slide label
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Artifacts (tissue/cellular structure that are created by preparative technique for histological specimen
Unavoidable
fixation by nature, kills cell, stabilize dynamic cell processes
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intentional
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pink and purple colour of H&E staining can become so familiar that they appear "normal", as if they were the natural color of tissue
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