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Revision (Haemotology (investigations (FBC (RBC, plasma, haemoglobin,…
Revision
Haemotology
investigations
bone marrow assessment
variety of stains
hematoxylin + eosin
reticulin
romanowsky
assess haemopoiesis
production of RBC + platelets
in bone marrow
erythrocyte sedimentation rate
marker for inflammation
rate = RBC sediment into vessels
blood film
number of cells
characteristics
morphology
romanowsky stain
methylene blue
eosin Y
FBC
RBC
plasma
haemoglobin
platelets
WBC
cells displace diluent = increase resistance
change in potential + duration measured
light scattered according to vol/SA/complexity
haematinic assays
markers
iron
B12
folate
ferritin
identifies nutrient causes of anaemia
infections
malaria
worms
Paul Bunnell test
detects infection of B-lymphocytes
via reaction of PB antigen + heterophile antigen
immunophenotyping
measures protein antigens on CS
identifies unusual cells
uses IgG antibodies to attack CD-antigens
haemoglobin + variant detection
differences in molecular structure of haemoglobin
blood components
whole blood = anticoagulant
serum = no anticoagulant
plasma = anticoagulant
study of blood
quantitive measurements = numerical
cellular constituents
measure of cells/enzymes/proteins
qualitative measurements = appearance/function
haemoglobin
indicator of anaemia
blood film
:
Histology
Immunology
Biochemistry