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METHODS (Molecular biology (Transformation (cells stored -80, Ref cell…

- - - - 135V 15 min
      - TAE
- - - - BioRad instructions
      - V min
      - Diagram of sandwich
    - - block
      - primary abs
      - secondary abs
      - Washes
    - - Substrates
        
        Table of strengths
      - LI-CORE oddessy Fc
        
        Channels, lengths
    - - Image Studio Lite
      - Excel
      - Graph Pad Prism
- - - - wash in PBS, add PBS-glycine
      - wash with PBS, add PFA
    - - triton-X-100 in BSA
      - make up 0.5mL ab in BSA
      - lay coverslip on 100 uL of ab, leave for 1 hour (repeat with secondary)
      - mount with 30 uL of fluoromount + DAPI
    - - Confocal 5 microscope