Please enable JavaScript.

Coggle requires JavaScript to display documents.

Lec 17: Sectioning (Procedure (2 different methods of Attaching or…

- - - - 1) glycerin-albumin (an adhesive) rubbed on one surface of a glass slide
      - 2) A drop or two of distilled water is then placed on the slide
      - 3) ribbon containing the tissue are floated on the water on the slide
      - 4) The slide is placed on a slide warming plate (45 - 50C) so as to soften the paraffin and flatten out the folds
      - 5) air dried to firmly adhere tissue sections to the glass slide
    - - 1) float the paraffin ribbon containing the tissue sections onto the surface of a warm water bath (45- 50C) containing gelatin (an adhesive)
      - 2) The heat of the water bath softens the paraffin to remove folds in the tissue sections
      - 3) slides with attached section are placed on a slide warming plate
      - 4) The tissue sections are then air dried to firmly attach them to the glass