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Lec 15: Dehydration (Techniques (Duration of dehydration (Tissue blocks 1…
Lec 15: Dehydration
Why?
To remove water from tissues, and to replace with other solution
Tissues contain much "free" water which does not mix well with the paraffin used later in the procedure. Thus it must undergo alcohol dehydration
Materials are to be embedded in paraffin to facilitate microtomy and Paraffin was insoluble in water (hydrophobic) interfere microtomy
Most of the stains are used as alcoholic solutions, present of water might interfere staining process
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