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CELL DISRUPTION (1) NON MECHANICAL CELL DISRUPTION (d) ACID ALKALI (eg:…
CELL DISRUPTION
INTRODUCTION
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Types of Cell:
Broken readily by gentle treatment and osmotic shock. Example: animal cells and some gram-negative bacteria
Highly resistant to breakage. Example: yeast, green algae, fungal mycelia and some gram-positive bacteria
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Method of agitation of cell suspension using small steel or glass beads with size about 0.2-1.0 mm diameter broken down by high liquid shear gradient and collision with the beads.
The rate of effectiveness of product release can be modified by changing the rates of agitation and the size of the beads, and the dimension of the equipment .
larger number of small beads are more effective than larger beads because of the increased of collision between beads and cell.
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hughes press and X-press enable frozen cell pastes to be forced under high pressure :150-230 MPa , 10-15 tons per square inch through narrow orifices, the disruption being produced by phase and volume changes and by slid shear due to the ice crystals
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Cell lysis or cellular distruption is a cell biology method for the release of biological molecules including organelles, proteins, DNA, RNA and lipids from inside a cell
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Method that use pulsed, high frequency sound waves to break cells. Best suit to volume less than 100 ml and not suitable to use in enzyme industries.
Method that use high pressure to break the cell, for example Manton-Gaulin APV type homogeizer. A good unicellular organisms disruption method provided enzymes needed are not heat labile. The method productivity highly affected by number of passage of sample into homogenizer.
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