CHAPTER 10, 11, 12
Photosynthesis
Calvin Cycle
Chloroplast
Light Capturing Reactions
USES: Water and light
PRODUCES: Oxygen
USES: Carbon Dioxide
PRODUCES: Sugar and 3RuBP
Thylakoid: Has ETC and ATP synthase for photo-phosphorylation
Granum: Stack of thylakoid
Stroma: Space inside chloroplast
Pigment: Molecules that absorb certain wavelengths
Chlorophyll b: Absorbs light
Carotenoids/Carotene: Absorb/pass energy to chlorophyll
Light
Blue light excites electrons better than red light
3 possible fates for electrons when absorbed
More Terms
Antenna Complex: Group of chlorophyll molecules and accessory pigments by an array of proteins
Energy is transferred to nearby pigments
Reduction/Oxidation = electrons are transferred to a new compound/acceptor
Emission of heat/light
Resonance Energy Transfer: Pigments in an antenna complex absorb photons from light, the energy (but not the photon itself) is passed to a nearby chlorophyll molecules, where another electron is excited in response to it
Reaction Center: Photons are absorbed by chlorophyll molecules
Photosystem
Photosystem I
Photosystem II
STEPS
End of photosystem I
Electrons are excited in reaction center chlorophyll molecules
The reaction center pigments are oxidized, and the excited electrons are passed through a series of carriers inside the photosystem, then to a molecule called ferredoxin, and then to the enzyme NADP+
Pigments in the antenna complex absorb photons and pass the energy to the photosystem I reaction center
NADP+ reductase transfers 2 electrons and a proton to reduce NADP+ and form NADPH
Final Electron Acceptor: NADP+
Molecule Produced When Electron Accepted: NADPH
Pheophytin
Water donates electrons to photosystem II
accepts excited electron from chlorophyll
Key to transforming light energy to chemical energy
Same as chlorophyll but lacks mg atom in the head region
OCCURS FIRST in photosystems
Z-Scheme: A model for how Photosystems II and I interact
STEPS
1. Carbon Fixation
The process converts CO2 into a more biologically useful form, carbon in the compound is reduced by attaching it to another carbon
Addition of CO2 to an organic compound
2. Reduction
3.Regeneration of RuBP: ATP is reduced to ADP
6 NADPH are reduced to NAD and H
Sugar is also produced here
How is it Regulated?
When sugar supplies are high, the production of proteins required for photosynthesis is inhibited, but the production of proteins required to process and store sugars is stimulated
Rubisco is activated by regulatory molecules that are produced when light is available, but inhibited in conditions of low CO2 availability – when photorespiration is favored
Presence of light triggers the production of proteins required for photosynthesis
Stomata
Pores away from cuticles that get CO2 in and oxygen out of cells
Open during day time
Cuticles:Prevents water evaporating out of tissues, prevents transport of gases like CO2 and oxygen
Closed when too hot or nightime
Maximizing CO2 Concentration
CAM = Crassulacean Acid Metabolism
C4 Pathway
Acts as additional fixation step
When fixing CO2 produces 4-carbon molecule than a 3-carbon molecule
Beneficial in conditions to space
Beneficial in condition to time
Same as C4 but during the nighttime and the products are then used during the day in the calvin cycle
Sugars
SUCROSE: when photosynthesis is done slowly
STARCH: when photosynthesis is done fast
GLUCOSE: gluconeogenesis
Cell-Cell Interaction
Extracellular material
Cross-linked network of long filaments in a stiff surrounding material
FUNCTION: Defines cell shape and attches it to another cell/acts a first-line defense against the outside world. Withstands compression and straining
Ground substance: concrete gel mixture, withstands pressure
Filaments: longs rods that resist pulling and pushing
Extracellular Matrix: Fiber composite secretion, provides structural support
Animals = more protein than carbohydrate
Plants = more carbohydrate than protein
Made of collagen protein and ground substance Proteoglycan, fibronetin
Cell Wall in Plants
Turgor Pressure
Pectin
Secondary Cell Wall: made when cells matures and stops growing
When incoming water pushes the plasma membrane against the cell wall
In normal conditions: the concentration of solutes is higher inside the cell than outside, causing water to enter the cell via osmosis
Made in the rough ER and Golgi
It’s hydrophilic and attracts/hold large amounts of water
Gaps
Small molecules go through these gaps between cells
Activate/inactivate proteins that already exist in the cell that are involved with metabolism, membrane transport, secretion, and the cytoskeleton
Gene expression, altering which proteins are produced and which are not
PLANTS: Plasmodesmata
ANIMALS: gap junction
Cell-Cell Communication
Lipid Molecules
SOLUBLE: Can diffuse across hydrophobic regions, enter cytosol, INSIDE THE CELL
INSOLUBLE: Cannot cross membrane, INSIDE PLASMA MEMBRANE
Signal Deactivation
Crosstalk
Phosphatases: This enzyme removes phosphate groups from necessary components of these signal-receptor reactions
Second Messenger Concentration: Second messengers are converted into storage molecules through pumps in the ER of the cytosol
GTP Hydrolysis: The hydrolysis of GTP to GDP makes the G protein inactive and therefore production of second messengers are stopped
Second messenger CAN STIMULATE 2nd pathway
Second messenger IS STIMULATED by 2nd pathway
Second messenger CAN INHIBIT 2nd pathway
For Prokaryotic
Quorum Sensing
Use of signal pathways that respond to population density
Cell Cycle
Middle Lamella
Allows plant cells to stick to each other, top layer of cell