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Laboratory Techniques (Physical ((Distillation (Seperation process that…
Laboratory Techniques
Physical
Distillation
Seperation process that involves heating a liquid to its boiling point, transferring the vapour to a different portion of the apparatus.
Filtration
The process in which solid particles in a liquid or gaseous fluid are removed by the use of a filter.
Centrifugation
Seperation process which uses the action of centrifugal force to promote accelerated setting of particles in a solid-liquid mixture.
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Chromatography
An analytical technique commonly used for seperating a misture of chemical substances into its individual components.
Weighing
To determine the force that gravitation exerts upon by the use of a balance, scale, or an another mechanical device
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Chemical/Biological
Autoradiography
This technique is used for study the steps and location of synthesis of molecules and to trace metabolic events in the cell.
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Cytochemical Methods
These methods are used to locate specific chemical constituents within the cells by
differentiating a particular part from other parts by colouring them with a specific stain or dye.
Cell fractionation
In this method different organelles of cells such as nucleus, mitochondria, ribosomes etc. having different particle size and weight are seperated by rotating them in a centrifuge in different speeds.
Culturing of Bacteria
To obtain well-isolated colonies, it is essential to disperse the inoclum on the surface of an enriched agar plate so that individual bacteria are well seperated from each other.
Ultracentrifugation
By rotation at a high speed, particles/organelles of different sizes and shapes to seperate them according to their density.
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Tissue Culture
This technique involves growing living cells outside the organism ny providing all necessary conditions for their survival and growth
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